Hi SungYun, Yes, if you want to connect -50V to VSUB, you have to short 1-2 pins of J_SUB. The power up sequence is -50V -> 3.3V -> 1.8V -> +/-5V -> +/-10V -> +50V. The power down sequence is +50V -> +/-10V -> +/-5V -> 1.8V -> 3.3V -> -50V. You don't need to connect the FPGA during power up. Regards, Charles
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